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KMID : 0545119940040020108
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 2 p.108 ~ p.112
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Genetic Organization of the Recombinant Bacillus pasteurii Urease Genes Expressed in Escherichia coli
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KIM SANG-DAL
ROBERT P. HAUSINGER
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Abstract
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The genetic organization of the urease gene cluster from an alkalophilic Bacillus pasteurii was determined by subcloning and Tn5 transposon mutagenesis of a 10.7 kilobasepair cloned fragment A region of DNA between 5.0 and 6.0 kb in length is necessary for urease activity. In vitro transcription-translation analysis of transposon insertion mutants of the cloned urease genes demonstrated that the major (M_r 67,000) and minor (M_r 20,000) structural peptides of urease are encoded at one end of the urease gene cluster and at least 3 additional polypeptides are encoded by adjacent DNA sequences.
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KEYWORD
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